Exhibit 21.1

Subsidiary

Jurisdiction of Organization

Athlinks Inc.

Delaware

ChronoTrack Administrator LLC

Delaware

ChronoTrack Holdings LLC

Delaware

ChronoTrack Mexico, S. DE R.I. DE C.V.

Mexico

ChronoTrack Systems Europe B.V.

Netherlands

Healthy Way of Life I, LLC

Delaware

Healthy Way of Life II, LLC

Delaware

Healthy Way of Life III, LLC

Delaware

Healthy Way of Life IV, LLC

Delaware

Healthy Way of Life V, LLC

Delaware

Healthy Way of Life VI, LLC

Delaware

Healthy Way of Life VII, LLC

Delaware

Healthy Way of Life VIII, LLC

Delaware

Healthy Way of Life IX, LLC

Delaware

Healthy Way of Life X, LLC

Delaware

Healthy Way of Life XI, LLC

Delaware

Healthy Way of Life XII, LLC

Delaware

Healthy Way of Life XIII, LLC

Delaware

Healthy Way of Life XIV, LLC

Delaware

Healthy Way of Life XV, LLC

Delaware

Healthy Way of Life XVI, LLC

Delaware

Healthy Way of Life XVII, LLC

Delaware

Healthy Way of Life XVIII, LLC

Delaware

Healthy Way of Life XIX, LLC

Delaware

Healthy Way of Life XX, LLC

Delaware

Life Time, Inc.

Minnesota

Life Time Captive Insurance Company

Vermont

Life Time Digital, LLC

Delaware

Life Time Foundation

Minnesota

Life Time LL, LLC

Delaware

Life Time Mixed Use Management Services Company, LLC

Delaware

Life Time Mixed Use Real Estate Company, LLC

Delaware

Life Time Real Estate Development & Construction, LLC

Delaware

LT Canada Co-Borrower GP Inc.

Ontario, Canada

LT Canada Co-Borrower Holdco, LP

Ontario, Canada

LT Canada Co-Borrower, LP

Ontario, Canada

LT Co-Borrower, LLC

Delaware

LT Co-Borrower Holdco, LLC

Delaware

LT Headquarters 2, LLC

Delaware

LT Living (Dallas) Managing Member, LLC

Delaware

LT Living (Dallas) Property, LLC

Delaware

LT Mixed Use (Burlington), LLC

Delaware

LT Mixed Use (Dallas-Montfort), LLC

Delaware

LT Mixed Use (Edina), LLC

Delaware

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LT Mixed Use (Paradise Valley), LLC

Delaware

LT Mixed Use (Raleigh), LLC

Delaware

LT Mixed Use (Stevens Creek), LLC

Delaware

LT Mixed Use ReCo (Henderson), LLC

Delaware

LT MRS Holdings, LLC

Delaware

LT (Connecticut) Property Management Company, LLC

Delaware

LT (Florida) Property Management Company, LLC

Delaware

LT (Illinois) Property Management Company, LLC

Delaware

LT (Massachusetts) Property Management Company, LLC

Delaware

LT (Nevada) Management Services Company, LLC

Delaware

LT (Nevada) Property Management Company, LLC

Delaware

LTF Architecture, LLC

Delaware

LTF Intermediate Holdings, Inc.

Delaware

LTF Club Management Company, LLC

Delaware

LTF Club Operations Company, Inc.

Minnesota

LTF Club Operations Company Canada Inc.

Ontario, Canada

LTF Club Operations Company Kansas, Inc.

Kansas

LTF Construction Company, LLC

Delaware

LTF Educational Programs, LLC

Delaware

LTF Ground Lease Company, LLC

Delaware

LTF Lease Company, LLC

Delaware

LTF Lease Company (Manhattan Four), LLC

Delaware

LTF Management Services, LLC

Delaware

LTF Maryland Liquor, LLC

Maryland

LTF Operations Holdings, Inc.

Minnesota

LTF Real Estate CMBS II, LLC

Delaware

LTF Real Estate Company, Inc.

Minnesota

LTF Real Estate Holdings, LLC

Delaware

LTF Real Estate MP I, LLC

Delaware

LTF Real Estate MP II, LLC

Delaware

LTF Real Estate MP III, LLC

Delaware

LTF Restaurant Company, LLC

Delaware

LTF Triathlon Series, LLC

Delaware

LTF Yoga Company, LLC

Delaware

Massage Retreat & Spa, Inc.

Delaware

Massage Retreat & Spa Franchise Company L.L.C.

Minnesota